LOCUS             ZENUB1REV.        6114 BP SS-DNA              SYN 11-DEC-2008
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
FEATURES             Location/Qualifiers
     frag            1..>6113
                     /note="1 to 6114 of pORI.Z.inv EnUb1(ZEN-Ub1rev) "
     frag            join(1..>3386,<3387..>3424,complement(<3431..>3439),
                     complement(<4835..>6092),<6093..>6113)
                     /note="2294 to 8407 of pZEN-UB1 [Split]"
     CDS             <1..>174
                     /note="lacZ N-ter. (modified) [Split]"
     primer_bind     complement(111..132)
                     /note="LacZ.SEE.rc"
     DNA             complement(<175..>3354)
                     /note="LACZ(for recom.) "
     CDS             <175..3075
                     /note="LacZ"
     primer_bind     complement(320..342)
                     /note="KOVp.neo3'-5'.8 primer (Tm 60¡C)"
     primer_bind     2975..2993
                     /note="LacZ.DSW.fw"
     primer_bind     3025..3051
                     /note="LacZ.YQL"
     primer_bind     3079..3096
                     /note="LacZ fw8"
     frag            3106..>3354
                     /note="SV40-Poly A Signal"
     frag            3399..3424
                     /note="I-CeuI"
     insertion_seq   join(complement(<3431..>3439),<6093..>6113)
                     /note="NheI Adaptor [Split]"
     frag            join(complement(<3431..>3439),<6093..>6113)
                     /note="7485 to 7565 of pZEN-SV1/ZEN9(SV40pr)Clone#2 [Split]
     misc_recomb     complement(3440..3473)
                     /note="loxP (3')"
     frag            complement(3440..4834)
                     /note="6280 to 7674 of pZEN6"
     polyA_site      complement(<3479..3963)
                     /note="PGK.poly A "
     frag            join(complement(3479..>3479),complement(<3481..3963))
                     /note="poly A  [Split]"
     primer_bind     complement(3513..3534)
                     /note="pGK.Neo.2fw"
     primer          complement(3513..3534)
                     /note="pGK.Neo.2fw"
     primer          complement(3687..3711)
                     /note="PGK.polya.3'fw5"
     primer          complement(3687..3711)
                     /note="PGK.polya.3'fw1"
     primer          3843..3862
                     /note="PGK.polyA.1rc"
     primer          3896..3914
                     /note="Neo.6rc"
     CDS             complement(3964..>4767)
                     /note="neo"
     mutation        complement(4213..4215)
                     /note="D"
     primer          complement(4269..4286)
                     /note="Neo7. fw"
     primer          4432..4450
                     /note="Neo.4rc"
     promoter        complement(4768..4834)
                     /note="EM7 promoter"
     exon            complement(<4835..4837)
                     /note="5' end of exon 2"
     frag            complement(4835..6047)
                     /note="9971 to 11183 of pRosaCRE.hUB-lacZ(1208)"
     frag            complement(<4835..>6047)
                     /note="1 to 1223 of hUB promoter (Fse/Age) [Split]"
     promoter        complement(4835..6047)
                     /note="hUBp (human Ubiquitin promoter)"
     primer          4855..4875
                     /note="hUbi.pr.rc1"
     primer          complement(4949..4966)
                     /note="hUbi.pr.fw3"
     primer          4988..5005
                     /note="hUB.seq5-3' (Tm = 48¡C)"
     primer          complement(5301..5321)
                     /note="hUB.seq4-5' (Tm = 50¡C)"
     primer          5349..5366
                     /note="hUB.seq3-3' (Tm = 51¡C)"
     primer          complement(5351..5375)
                     /note="hUBiprfw2"
     primer          5572..5592
                     /note="hUBi.pr.rc2"
     primer          5652..5669
                     /note="hUB.seq1-3' (Tm = 48¡C)"
     primer          complement(5654..5672)
                     /note="hUB.seq2-5' (Tm = 51¡C)"
     misc_feature    complement(5713..5713)
                     /note="transcription start site"
     TATA_signal     complement(5738..5745)
                     /note="TATA box"
     protein_bind    complement(5759..5764)
                     /note="Sp 1 binding site"
     primer          complement(5800..5818)
                     /note="hUBifw1"
     primer          5895..5915
                     /note="hUB.seq7-3' (Tm = 52¡C)"
     primer          complement(5895..5915)
                     /note="hUB.seq6-5' (Tm = 52¡C)"
     primer_bind     5895..5915
                     /note="hUB.seq7-3'"
     protein_bind    complement(5943..5948)
                     /note="Sp 1 binding site"
     misc_recomb     complement(6054..6087)
                     /note="LoxP (5')"
BASE COUNT      1491 A      1723 C      1598 G      1302 T         0 OTHER
ORIGIN      ?
        1 GGTACCGATT TAAATGATCC AGTGGTCCTG CAGAGGAGAG ATTGGGAGAA TCCCGGTGTG
       61 ACACAGCTGA ACAGACTAGC CGCCCACCCT CCCTTTGCTT CTTGGAGAAA CAGTGAGGAA
      121 GCTAGGACAG ACAGACCAAG CCAGCAACTC AGATCTTTGA ACGGGGAGTG GAGATTTGCC
      181 TGGTTTCCGG CACCAGAAGC GGTGCCGGAA AGCTGGCTGG AGTGCGATCT TCCTGAGGCC
      241 GATACTGTCG TCGTCCCCTC AAACTGGCAG ATGCACGGTT ACGATGCGCC CATCTACACC
      301 AACGTGACCT ATCCCATTAC GGTCAATCCG CCGTTTGTTC CCACGGAGAA TCCGACGGGT
      361 TGTTACTCGC TCACATTTAA TGTTGATGAA AGCTGGCTAC AGGAAGGCCA GACGCGAATT
      421 ATTTTTGATG GCGTTAACTC GGCGTTTCAT CTGTGGTGCA ACGGGCGCTG GGTCGGTTAC
      481 GGCCAGGACA GTCGTTTGCC GTCTGAATTT GACCTGAGCG CATTTTTACG CGCCGGAGAA
      541 AACCGCCTCG CGGTGATGGT GCTGCGCTGG AGTGACGGCA GTTATCTGGA AGATCAGGAT
      601 ATGTGGCGGA TGAGCGGCAT TTTCCGTGAC GTCTCGTTGC TGCATAAACC GACTACACAA
      661 ATCAGCGATT TCCATGTTGC CACTCGCTTT AATGATGATT TCAGCCGCGC TGTACTGGAG
      721 GCTGAAGTTC AGATGTGCGG CGAGTTGCGT GACTACCTAC GGGTAACAGT TTCTTTATGG
      781 CAGGGTGAAA CGCAGGTCGC CAGCGGCACC GCGCCTTTCG GCGGTGAAAT TATCGATGAG
      841 CGTGGTGGTT ATGCCGATCG CGTCACACTA CGTCTGAACG TCGAAAACCC GAAACTGTGG
      901 AGCGCCGAAA TCCCGAATCT CTATCGTGCG GTGGTTGAAC TGCACACCGC CGACGGCACG
      961 CTGATTGAAG CAGAAGCCTG CGATGTCGGT TTCCGCGAGG TGCGGATTGA AAATGGTCTG
     1021 CTGCTGCTGA ACGGCAAGCC GTTGCTGATT CGAGGCGTTA ACCGTCACGA GCATCATCCT
     1081 CTGCATGGTC AGGTCATGGA TGAGCAGACG ATGGTGCAGG ATATCCTGCT GATGAAGCAG
     1141 AACAACTTTA ACGCCGTGCG CTGTTCGCAT TATCCGAACC ATCCGCTGTG GTACACGCTG
     1201 TGCGACCGCT ACGGCCTGTA TGTGGTGGAT GAAGCCAATA TTGAAACCCA CGGCATGGTG
     1261 CCAATGAATC GTCTGACCGA TGATCCGCGC TGGCTACCGG CGATGAGCGA ACGCGTAACG
     1321 CGAATGGTGC AGCGCGATCG TAATCACCCG AGTGTGATCA TCTGGTCGCT GGGGAATGAA
     1381 TCAGGCCACG GCGCTAATCA CGACGCGCTG TATCGCTGGA TCAAATCTGT CGATCCTTCC
     1441 CGCCCGGTGC AGTATGAAGG CGGCGGAGCC GACACCACGG CCACCGATAT TATTTGCCCG
     1501 ATGTACGCGC GCGTGGATGA AGACCAGCCC TTCCCGGCTG TGCCGAAATG GTCCATCAAA
     1561 AAATGGCTTT CGCTACCTGG AGAGACGCGC CCGCTGATCC TTTGCGAATA CGCCCACGCG
     1621 ATGGGTAACA GTCTTGGCGG TTTCGCTAAA TACTGGCAGG CGTTTCGTCA GTATCCCCGT
     1681 TTACAGGGCG GCTTCGTCTG GGACTGGGTG GATCAGTCGC TGATTAAATA TGATGAAAAC
     1741 GGCAACCCGT GGTCGGCTTA CGGCGGTGAT TTTGGCGATA CGCCGAACGA TCGCCAGTTC
     1801 TGTATGAACG GTCTGGTCTT TGCCGACCGC ACGCCGCATC CAGCGCTGAC GGAAGCAAAA
     1861 CACCAGCAGC AGTTTTTCCA GTTCCGTTTA TCCGGGCAAA CCATCGAAGT GACCAGCGAA
     1921 TACCTGTTCC GTCATAGCGA TAACGAGCTC CTGCACTGGA TGGTGGCGCT GGATGGTAAG
     1981 CCGCTGGCAA GCGGTGAAGT GCCTCTGGAT GTCGCTCCAC AAGGTAAACA GTTGATTGAA
     2041 CTGCCTGAAC TACCGCAGCC GGAGAGCGCC GGGCAACTCT GGCTCACAGT ACGCGTAGTG
     2101 CAACCGAACG CGACCGCATG GTCAGAAGCC GGGCACATCA GCGCCTGGCA GCAGTGGCGT
     2161 CTGGCGGAAA ACCTCAGTGT GACGCTCCCC GCCGCGTCCC ACGCCATCCC GCATCTGACC
     2221 ACCAGCGAAA TGGATTTTTG CATCGAGCTG GGTAATAAGC GTTGGCAATT TAACCGCCAG
     2281 TCAGGCTTTC TTTCACAGAT GTGGATTGGC GATAAAAAAC AACTGCTGAC GCCGCTGCGC
     2341 GATCAGTTCA CCCGTGCACC GCTGGATAAC GACATTGGCG TAAGTGAAGC GACCCGCATT
     2401 GACCCTAACG CCTGGGTCGA ACGCTGGAAG GCGGCGGGCC ATTACCAGGC CGAAGCAGCG
     2461 TTGTTGCAGT GCACGGCAGA TACACTTGCT GATGCGGTGC TGATTACGAC CGCTCACGCG
     2521 TGGCAGCATC AGGGGAAAAC CTTATTTATC AGCCGGAAAA CCTACCGGAT TGATGGTAGT
     2581 GGTCAAATGG CGATTACCGT TGATGTTGAA GTGGCGAGCG ATACACCGCA TCCGGCGCGG
     2641 ATTGGCCTGA ACTGCCAGCT GGCGCAGGTA GCAGAGCGGG TAAACTGGCT CGGATTAGGG
     2701 CCGCAAGAAA ACTATCCCGA CCGCCTTACT GCCGCCTGTT TTGACCGCTG GGATCTGCCA
     2761 TTGTCAGACA TGTATACCCC GTACGTCTTC CCGAGCGAAA ACGGTCTGCG CTGCGGGACG
     2821 CGCGAATTGA ATTATGGCCC ACACCAGTGG CGCGGCGACT TCCAGTTCAA CATCAGCCGC
     2881 TACAGTCAAC AGCAACTGAT GGAAACCAGC CATCGCCATC TGCTGCACGC GGAAGAAGGC
     2941 ACATGGCTGA ATATCGACGG TTTCCATATG GGGATTGGTG GCGACGACTC CTGGAGCCCG
     3001 TCAGTATCGG CGGAATTCCA GCTGAGCGCC GGTCGCTACC ATTACCAGTT GGTCTGGTGT
     3061 CAAAAATAAT AATAACCGGG CAGGGGGGAT CTAAGCTCTA GATAAGTAAT GATCATAATC
     3121 AGCCATATCA CATCTGTAGA GGTTTTACTT GCTTTAAAAA ACCTCCCACA CCTCCCCCTG
     3181 AACCTGAAAC ATAAAATGAA TGCAATTGTT GTTGTTAACT TGTTTATTGC AGCTTATAAT
     3241 GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT
     3301 TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGGAT CCCCCGGCTA
     3361 GAGTTTAAAC ACTAGAACTA GTGGATCCCC GGGCTCGATA ACTATAACGG TCCTAAGGTA
     3421 GCGACTCGAC CAACCTAGGA TAACTTCGTA TAGCATACAT TATACGAAGT TATCTAGGGG
     3481 CTGCAGGTCG AGGTCTGATG GAATTAGAAC TTGGCAAAAC AATACTGAGA ATGAAGTGTA
     3541 TGTGGAACAG AGGCTGCTGA TCTCGTTCTT CAGGCTATGA AACTGACACA TTTGGAAACC
     3601 ACAGTACTTA GAACCACAAA GTGGGAATCA AGAGAAAAAC AATGATCCCA CGAGAGATCT
     3661 ATAGATCTAT AGATCATGAG TGGGAGGAAT GAGCTGGCCC TTAATTTGGT TTTGCTTGTT
     3721 TAAATTATGA TATCCAACTA TGAAACATTA TCATAAAGCA ATAGTAAAGA GCCTTCAGTA
     3781 AAGAGCAGGC ATTTATCTAA TCCCACCCCA CCCCCACCCC CGTAGCTCCA ATCCTTCCAT
     3841 TCAAAATGTA GGTACTCTGT TCTCACCCTT CTTAACAAAG TATGACAGGA AAAACTTCCA
     3901 TTTTAGTGGA CATCTTTATT GTTTAATAGA TCATCAATTT CTGCAGACTT ACAGCGGATC
     3961 CCCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC
     4021 GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC
     4081 ACGGGTAGCC AACGCTATGT CCTGATAGCG GTCCGCCACA CCCAGCCGGC CACAGTCGAT
     4141 GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT
     4201 CACGACGAGA TCATCGCCGT CGGGCATGCG CGCCTTGAGC CTGGCGAACA GTTCGGCTGG
     4261 CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG
     4321 AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC
     4381 AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG
     4441 GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC
     4501 TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG
     4561 CCGCGCTGCC TCGTCCTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG
     4621 AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG
     4681 TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA
     4741 TCCATCTTGT TCAATGGCCG ATCCCATGGT TTAGTTCCTC ACCTTGTCGT ATTATACTAT
     4801 GCCGATATAC TATGCCGATG ATTAATTGTC AACACGTCTA ACAAAAAAGC CAAAAACGGC
     4861 CAGAATTTAG CGGACAATTT ACTAGTCTAA CACTGAAAAT TACATATTGA CCCAAATGAT
     4921 TACATTTCAA AAGGTGCCTA AAAAACTTCA CAAAACACAC TCGCCAACCC CGAGCGCATA
     4981 GTTCAAAACC GGAGCTTCAG CTACTTAAGA AGATAGGTAC ATAAAACCGA CCAAAGAAAC
     5041 TGACGCCTCA CTTATCCCTC CCCTCACCAG AGGTCCGGCG CCTGTCGATT CAGGAGAGCC
     5101 TACCCTAGGC CCGAACCCTG CGTCCTGCGA CGGAGAAAAG CCTACCGCAC ACCTACCGGC
     5161 AGGTGGCCCC ACCCTGCATT ATAAGCCAAC AGAACGGGTG ACGTCACGAC ACGACGAGGG
     5221 CGCGCGCTCC CAAAGGTACG GGTGCACTGC CCAACGGCAC CGCCATAACT GCCGCCCCCG
     5281 CAACAGACGA CAAACCGAGT TCTCCAGTCA GTGACAAACT TCACGTCAGG GTCCCCAGAT
     5341 GGTGCCCCAG CCCATCTCAC CCGAATAAGA GCTTTCCCGC ATTAGCGAAG GCCTCAAGAC
     5401 CTTGGGTTCT TGCCGCCCAC CATGCCCCCC ACCTTGTTTC AACGACCTCA CAGCCCGCCT
     5461 CACAAGCGTC TTCCATTCAA GACTCGGGAA CAGCCGCCAT TTTGCTGCGC TCCCCCCAAC
     5521 CCCCAGTTCA GGGCAACCTT GCTCGCGGAC CCAGACTACA GCCCTTGGCG GTCTCTCCAC
     5581 ACGCTTCCGT CCCACCGAGC GGCCCGGCGG CCACGAAAGC CCCGGCCAGC CCAGCAGCCC
     5641 GCTACTCACC AAGTGACGAT CACAGCGATC CACAAACAAG AACCGCGACC CAAATCCCGG
     5701 CTGCGACGGA ACTAGCTGTG CCACACCCGG CGCGTCCTTA TATAATCATC GGCGTTCACC
     5761 GCCCCACGGA GATCCCTCCG CAGAATCGCC GAGAAGGGAC TACTTTTCCT CGCCTGTTCC
     5821 GCTCTCTGGA AAGAAAACCA GTGCCCTAGA GTCACCCAAG TCCCGTCCTA AAATGTCCTT
     5881 CTGCTGATAC TGGGGTTCTA AGGCCGAGTC TTATGAGCAG CGGGCCGCTG TCCTGAGCGT
     5941 CCGGGCGGAA GGATCAGGAC GCTCGCTGCG CCCTTCGTCT GACGTGGCAG CGCTCGCCGT
     6001 GAGGAGGGGG GCGCCCGCGG GAGGCGCCAA AACCCGGCGC GGAGGCCATG CATATAACTT
     6061 CGTATAGCAT ACATTATACG AAGTTATCTC GACCACCGCG GGTTTAAAGC TAGG
//