LOCUS ZEN UBI HY 6306 BP SS-DNA SYN 08-FEB-2008 DEFINITION - ACCESSION - KEYWORDS - SOURCE - FEATURES Location/Qualifiers misc_structure 1..>6305 /note="CASSETTE" CDS <1..>174 /note="lacZ N-ter. (modified) [Split]" frag 1..>6305 /note="1 to 6306 of pORI.Zen-Ub1-Hyg(Nhe1)" frag join(1..>3386,<3387..>4683,<6302..>6305) /note="2294 to 8407 of pZEN-UB1 [Split]" primer_bind complement(111..132) /note="LacZ.SEE.rc" CDS <175..3075 /note="LacZ" DNA complement(<175..>3354) /note="LACZ(for recom.) " primer_bind complement(320..342) /note="KOVp.neo3'-5'.8 primer (Tm 60¡C)" primer_bind 2975..2993 /note="LacZ.DSW.fw" primer_bind 3025..3051 /note="LacZ.YQL" frag 3106..>3354 /note="Poly A Signal" frag 3399..3424 /note="I-CeuI" misc_recomb 3431..3464 /note="LoxP (5')" promoter 3471..4683 /note="hUBp (human Ubiquitin promoter)" frag 3471..4683 /note="9971 to 11183 of pRosaCRE.hUB-lacZ(1208)" frag <3471..>4683 /note="1 to 1223 of hUB promoter (Fse/Age) [Split]" protein_bind 3570..3575 /note="Sp 1 binding site" primer complement(3603..3623) /note="hUB.seq7-3' (Tm = 52¡C)" primer 3603..3623 /note="hUB.seq6-5' (Tm = 52¡C)" primer 3700..3718 /note="hUBifw1" protein_bind 3754..3759 /note="Sp 1 binding site" TATA_signal 3773..3780 /note="TATA box" misc_feature 3805..3805 /note="transcription start site" primer 3846..3864 /note="hUB.seq2-5' (Tm = 51¡C)" primer complement(3849..3866) /note="hUB.seq1-3' (Tm = 48¡C)" primer complement(3926..3946) /note="hUBi.pr.rc2" primer 4143..4167 /note="hUBiprfw2" primer complement(4152..4169) /note="hUB.seq3-3' (Tm = 51¡C)" primer 4197..4217 /note="hUB.seq4-5' (Tm = 50¡C)" primer complement(4513..4530) /note="hUB.seq5-3' (Tm = 48¡C)" primer 4552..4569 /note="hUbi.pr.fw3" primer complement(4643..4663) /note="hUbi.pr.rc1" exon 4681..>4683 /note="5' end of exon 2" frag join(4684..>4750,<5777..6300) /note="6280 to 7674 of pZEN6 [Split]" promoter 4684..4750 /note="EM7 promoter" misc_feature 4751..>5773 /note="hygro2 (has single point silent mutation that removes a BsmBI site)" CDS 4751..5773 /note="Hygro2" frag complement(<4751..>5776) /note="3390 to 4706 of pRX.i[(71)-(GFP-§pA)66](ÄUH2Ä) [Split]" frag 4751..5776 /note="60 to 1085 of HYG cassette (AseI-XmnI)" mutation 4816..4816 /note="C to G engineered mutation to remove BsmBI site" primer_bind 5125..5159 /note="Hyg.Mut.Asis1.fw" primer_bind 5656..5676 /note="HYG 3'end fw1" polyA_signal 5777..>6261 /note="PGK-poly A " primer_bind complement(5785..5808) /note="Hyg.3'UTR.rc1" primer_bind complement(5792..5820) /note="Hyg.3'UTR.rc2" primer complement(5826..5844) /note="Neo.6rc" primer complement(5878..5897) /note="PGK.polyA.1rc" primer 6029..6053 /note="PGK.polya.3'fw1" primer 6029..6053 /note="PGK.polya.3'fw5" primer 6206..6227 /note="pGK.Neo.2fw" misc_recomb 6267..6300 /note="loxP (3')" frag <6302..>6305 /note="7485 to 7565 of pZEN-SV1/ZEN9(SV40pr)Clone#2 [Split] BASE COUNT 1396 A 1587 C 1833 G 1490 T 0 OTHER ORIGIN ? 1 GGTACCGATT TAAATGATCC AGTGGTCCTG CAGAGGAGAG ATTGGGAGAA TCCCGGTGTG 61 ACACAGCTGA ACAGACTAGC CGCCCACCCT CCCTTTGCTT CTTGGAGAAA CAGTGAGGAA 121 GCTAGGACAG ACAGACCAAG CCAGCAACTC AGATCTTTGA ACGGGGAGTG GAGATTTGCC 181 TGGTTTCCGG CACCAGAAGC GGTGCCGGAA AGCTGGCTGG AGTGCGATCT TCCTGAGGCC 241 GATACTGTCG TCGTCCCCTC AAACTGGCAG ATGCACGGTT ACGATGCGCC CATCTACACC 301 AACGTGACCT ATCCCATTAC GGTCAATCCG CCGTTTGTTC CCACGGAGAA TCCGACGGGT 361 TGTTACTCGC TCACATTTAA TGTTGATGAA AGCTGGCTAC AGGAAGGCCA GACGCGAATT 421 ATTTTTGATG GCGTTAACTC GGCGTTTCAT CTGTGGTGCA ACGGGCGCTG GGTCGGTTAC 481 GGCCAGGACA GTCGTTTGCC GTCTGAATTT GACCTGAGCG CATTTTTACG CGCCGGAGAA 541 AACCGCCTCG CGGTGATGGT GCTGCGCTGG AGTGACGGCA GTTATCTGGA AGATCAGGAT 601 ATGTGGCGGA TGAGCGGCAT TTTCCGTGAC GTCTCGTTGC TGCATAAACC GACTACACAA 661 ATCAGCGATT TCCATGTTGC CACTCGCTTT AATGATGATT TCAGCCGCGC TGTACTGGAG 721 GCTGAAGTTC AGATGTGCGG CGAGTTGCGT GACTACCTAC GGGTAACAGT TTCTTTATGG 781 CAGGGTGAAA CGCAGGTCGC CAGCGGCACC GCGCCTTTCG GCGGTGAAAT TATCGATGAG 841 CGTGGTGGTT ATGCCGATCG CGTCACACTA CGTCTGAACG TCGAAAACCC GAAACTGTGG 901 AGCGCCGAAA TCCCGAATCT CTATCGTGCG GTGGTTGAAC TGCACACCGC CGACGGCACG 961 CTGATTGAAG CAGAAGCCTG CGATGTCGGT TTCCGCGAGG TGCGGATTGA AAATGGTCTG 1021 CTGCTGCTGA ACGGCAAGCC GTTGCTGATT CGAGGCGTTA ACCGTCACGA GCATCATCCT 1081 CTGCATGGTC AGGTCATGGA TGAGCAGACG ATGGTGCAGG ATATCCTGCT GATGAAGCAG 1141 AACAACTTTA ACGCCGTGCG CTGTTCGCAT TATCCGAACC ATCCGCTGTG GTACACGCTG 1201 TGCGACCGCT ACGGCCTGTA TGTGGTGGAT GAAGCCAATA TTGAAACCCA CGGCATGGTG 1261 CCAATGAATC GTCTGACCGA TGATCCGCGC TGGCTACCGG CGATGAGCGA ACGCGTAACG 1321 CGAATGGTGC AGCGCGATCG TAATCACCCG AGTGTGATCA TCTGGTCGCT GGGGAATGAA 1381 TCAGGCCACG GCGCTAATCA CGACGCGCTG TATCGCTGGA TCAAATCTGT CGATCCTTCC 1441 CGCCCGGTGC AGTATGAAGG CGGCGGAGCC GACACCACGG CCACCGATAT TATTTGCCCG 1501 ATGTACGCGC GCGTGGATGA AGACCAGCCC TTCCCGGCTG TGCCGAAATG GTCCATCAAA 1561 AAATGGCTTT CGCTACCTGG AGAGACGCGC CCGCTGATCC TTTGCGAATA CGCCCACGCG 1621 ATGGGTAACA GTCTTGGCGG TTTCGCTAAA TACTGGCAGG CGTTTCGTCA GTATCCCCGT 1681 TTACAGGGCG GCTTCGTCTG GGACTGGGTG GATCAGTCGC TGATTAAATA TGATGAAAAC 1741 GGCAACCCGT GGTCGGCTTA CGGCGGTGAT TTTGGCGATA CGCCGAACGA TCGCCAGTTC 1801 TGTATGAACG GTCTGGTCTT TGCCGACCGC ACGCCGCATC CAGCGCTGAC GGAAGCAAAA 1861 CACCAGCAGC AGTTTTTCCA GTTCCGTTTA TCCGGGCAAA CCATCGAAGT GACCAGCGAA 1921 TACCTGTTCC GTCATAGCGA TAACGAGCTC CTGCACTGGA TGGTGGCGCT GGATGGTAAG 1981 CCGCTGGCAA GCGGTGAAGT GCCTCTGGAT GTCGCTCCAC AAGGTAAACA GTTGATTGAA 2041 CTGCCTGAAC TACCGCAGCC GGAGAGCGCC GGGCAACTCT GGCTCACAGT ACGCGTAGTG 2101 CAACCGAACG CGACCGCATG GTCAGAAGCC GGGCACATCA GCGCCTGGCA GCAGTGGCGT 2161 CTGGCGGAAA ACCTCAGTGT GACGCTCCCC GCCGCGTCCC ACGCCATCCC GCATCTGACC 2221 ACCAGCGAAA TGGATTTTTG CATCGAGCTG GGTAATAAGC GTTGGCAATT TAACCGCCAG 2281 TCAGGCTTTC TTTCACAGAT GTGGATTGGC GATAAAAAAC AACTGCTGAC GCCGCTGCGC 2341 GATCAGTTCA CCCGTGCACC GCTGGATAAC GACATTGGCG TAAGTGAAGC GACCCGCATT 2401 GACCCTAACG CCTGGGTCGA ACGCTGGAAG GCGGCGGGCC ATTACCAGGC CGAAGCAGCG 2461 TTGTTGCAGT GCACGGCAGA TACACTTGCT GATGCGGTGC TGATTACGAC CGCTCACGCG 2521 TGGCAGCATC AGGGGAAAAC CTTATTTATC AGCCGGAAAA CCTACCGGAT TGATGGTAGT 2581 GGTCAAATGG CGATTACCGT TGATGTTGAA GTGGCGAGCG ATACACCGCA TCCGGCGCGG 2641 ATTGGCCTGA ACTGCCAGCT GGCGCAGGTA GCAGAGCGGG TAAACTGGCT CGGATTAGGG 2701 CCGCAAGAAA ACTATCCCGA CCGCCTTACT GCCGCCTGTT TTGACCGCTG GGATCTGCCA 2761 TTGTCAGACA TGTATACCCC GTACGTCTTC CCGAGCGAAA ACGGTCTGCG CTGCGGGACG 2821 CGCGAATTGA ATTATGGCCC ACACCAGTGG CGCGGCGACT TCCAGTTCAA CATCAGCCGC 2881 TACAGTCAAC AGCAACTGAT GGAAACCAGC CATCGCCATC TGCTGCACGC GGAAGAAGGC 2941 ACATGGCTGA ATATCGACGG TTTCCATATG GGGATTGGTG GCGACGACTC CTGGAGCCCG 3001 TCAGTATCGG CGGAATTCCA GCTGAGCGCC GGTCGCTACC ATTACCAGTT GGTCTGGTGT 3061 CAAAAATAAT AATAACCGGG CAGGGGGGAT CTAAGCTCTA GATAAGTAAT GATCATAATC 3121 AGCCATATCA CATCTGTAGA GGTTTTACTT GCTTTAAAAA ACCTCCCACA CCTCCCCCTG 3181 AACCTGAAAC ATAAAATGAA TGCAATTGTT GTTGTTAACT TGTTTATTGC AGCTTATAAT 3241 GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT 3301 TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGGAT CCCCCGGCTA 3361 GAGTTTAAAC ACTAGAACTA GTGGATCCCC GGGCTCGATA ACTATAACGG TCCTAAGGTA 3421 GCGACTCGAG ATAACTTCGT ATAATGTATG CTATACGAAG TTATATGCAT GGCCTCCGCG 3481 CCGGGTTTTG GCGCCTCCCG CGGGCGCCCC CCTCCTCACG GCGAGCGCTG CCACGTCAGA 3541 CGAAGGGCGC AGCGAGCGTC CTGATCCTTC CGCCCGGACG CTCAGGACAG CGGCCCGCTG 3601 CTCATAAGAC TCGGCCTTAG AACCCCAGTA TCAGCAGAAG GACATTTTAG GACGGGACTT 3661 GGGTGACTCT AGGGCACTGG TTTTCTTTCC AGAGAGCGGA ACAGGCGAGG AAAAGTAGTC 3721 CCTTCTCGGC GATTCTGCGG AGGGATCTCC GTGGGGCGGT GAACGCCGAT GATTATATAA 3781 GGACGCGCCG GGTGTGGCAC AGCTAGTTCC GTCGCAGCCG GGATTTGGGT CGCGGTTCTT 3841 GTTTGTGGAT CGCTGTGATC GTCACTTGGT GAGTAGCGGG CTGCTGGGCT GGCCGGGGCT 3901 TTCGTGGCCG CCGGGCCGCT CGGTGGGACG GAAGCGTGTG GAGAGACCGC CAAGGGCTGT 3961 AGTCTGGGTC CGCGAGCAAG GTTGCCCTGA ACTGGGGGTT GGGGGGAGCG CAGCAAAATG 4021 GCGGCTGTTC CCGAGTCTTG AATGGAAGAC GCTTGTGAGG CGGGCTGTGA GGTCGTTGAA 4081 ACAAGGTGGG GGGCATGGTG GGCGGCAAGA ACCCAAGGTC TTGAGGCCTT CGCTAATGCG 4141 GGAAAGCTCT TATTCGGGTG AGATGGGCTG GGGCACCATC TGGGGACCCT GACGTGAAGT 4201 TTGTCACTGA CTGGAGAACT CGGTTTGTCG TCTGTTGCGG GGGCGGCAGT TATGGCGGTG 4261 CCGTTGGGCA GTGCACCCGT ACCTTTGGGA GCGCGCGCCC TCGTCGTGTC GTGACGTCAC 4321 CCGTTCTGTT GGCTTATAAT GCAGGGTGGG GCCACCTGCC GGTAGGTGTG CGGTAGGCTT 4381 TTCTCCGTCG CAGGACGCAG GGTTCGGGCC TAGGGTAGGC TCTCCTGAAT CGACAGGCGC 4441 CGGACCTCTG GTGAGGGGAG GGATAAGTGA GGCGTCAGTT TCTTTGGTCG GTTTTATGTA 4501 CCTATCTTCT TAAGTAGCTG AAGCTCCGGT TTTGAACTAT GCGCTCGGGG TTGGCGAGTG 4561 TGTTTTGTGA AGTTTTTTAG GCACCTTTTG AAATGTAATC ATTTGGGTCA ATATGTAATT 4621 TTCAGTGTTA GACTAGTAAA TTGTCCGCTA AATTCTGGCC GTTTTTGGCT TTTTTGTTAG 4681 ACGTGTTGAC AATTAATCAT CGGCATAGTA TATCGGCATA GTATAATACG ACAAGGTGAG 4741 GAACTAAACC ATGAAAAAGC CTGAACTCAC CGCGACGTCT GTCGAGAAGT TTCTGATCGA 4801 AAAGTTCGAC AGCGTGTCCG ACCTGATGCA GCTCTCGGAG GGCGAAGAAT CTCGTGCTTT 4861 CAGCTTCGAT GTAGGAGGGC GTGGATATGT CCTGCGGGTA AATAGCTGCG CCGATGGTTT 4921 CTACAAAGAT CGTTATGTTT ATCGGCACTT TGCATCGGCC GCGCTCCCGA TTCCGGAAGT 4981 GCTTGACATT GGGGAATTCA GCGAGAGCCT GACCTATTGC ATCTCCCGCC GTGCACAGGG 5041 TGTCACGTTG CAAGACCTGC CTGAAACCGA ACTGCCCGCT GTTCTGCAGC CGGTCGCGGA 5101 GGCCATGGAT GCGATCGCTG CGGCCGATCT TAGCCAGACG AGCGGGTTCG GCCCATTCGG 5161 ACCGCAAGGA ATCGGTCAAT ACACTACATG GCGTGATTTC ATATGCGCGA TTGCTGATCC 5221 CCATGTGTAT CACTGGCAAA CTGTGATGGA CGACACCGTC AGTGCGTCCG TCGCGCAGGC 5281 TCTCGATGAG CTGATGCTTT GGGCCGAGGA CTGCCCCGAA GTCCGGCACC TCGTGCACGC 5341 GGATTTCGGC TCCAACAATG TCCTGACGGA CAATGGCCGC ATAACAGCGG TCATTGACTG 5401 GAGCGAGGCG ATGTTCGGGG ATTCCCAATA CGAGGTCGCC AACATCTTCT TCTGGAGGCC 5461 GTGGTTGGCT TGTATGGAGC AGCAGACGCG CTACTTCGAG CGGAGGCATC CGGAGCTTGC 5521 AGGATCGCCG CGGCTCCGGG CGTATATGCT CCGCATTGGT CTTGACCAAC TCTATCAGAG 5581 CTTGGTTGAC GGCAATTTCG ATGATGCAGC TTGGGCGCAG GGTCGATGCG ACGCAATCGT 5641 CCGATCCGGA GCCGGGACTG TCGGGCGTAC ACAAATCGCC CGCAGAAGCG CGGCCGTCTG 5701 GACCGATGGC TGTGTAGAAG TACTCGCCGA TAGTGGAAAC CGACGCCCCA GCACTCGTCC 5761 GAGGGCAAAG GAATAGGGGG ATCCGCTGTA AGTCTGCAGA AATTGATGAT CTATTAAACA 5821 ATAAAGATGT CCACTAAAAT GGAAGTTTTT CCTGTCATAC TTTGTTAAGA AGGGTGAGAA 5881 CAGAGTACCT ACATTTTGAA TGGAAGGATT GGAGCTACGG GGGTGGGGGT GGGGTGGGAT 5941 TAGATAAATG CCTGCTCTTT ACTGAAGGCT CTTTACTATT GCTTTATGAT AATGTTTCAT 6001 AGTTGGATAT CATAATTTAA ACAAGCAAAA CCAAATTAAG GGCCAGCTCA TTCCTCCCAC 6061 TCATGATCTA TAGATCTATA GATCTCTCGT GGGATCATTG TTTTTCTCTT GATTCCCACT 6121 TTGTGGTTCT AAGTACTGTG GTTTCCAAAT GTGTCAGTTT CATAGCCTGA AGAACGAGAT 6181 CAGCAGCCTC TGTTCCACAT ACACTTCATT CTCAGTATTG TTTTGCCAAG TTCTAATTCC 6241 ATCAGACCTC GACCTGCAGC CCCTAGATAA CTTCGTATAA TGTATGCTAT ACGAAGTTAT 6301 CCTAGG //