10000 Celsr1 Status: Cancelled
| Gene | Celsr1 |
| Chromosome | 15 |
| Deletion Start | 86,033,767 |
| Deletion End | 86,030,164 |
| Deletion Size | 3,604 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 86,033,425 | 86,033,445 | TACTAAGGGCACGCGCCTATG |
| TUP | 86,033,341 | 86,033,358 | TCCGCTCAGTTCCTGGAC |
| TUR | 86,033,319 | 86,033,337 | GGGAAGCAGAGCGCATCTC |
| TDF | 86,030,876 | 86,030,894 | GCCCACGTCTGGTGTGATC |
| TDP | 86,030,845 | 86,030,868 | CATTCTCTCTGTCCAGCCGGCGCT |
| TDR | 86,030,817 | 86,030,837 | GCGAGAGCCCAAAGGTTGTAC |
PCR Primers
| Primer | Sequence |
| SU | TGCTGCAGGTTGATGCGCG |
| SD | TGAAACAGCCCAGGCTTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 405 bp | - | + | + |
| TUF | TUR | 127 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | 322 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
