10004 Shisa2 Status: Cancelled
| Gene | Shisa2 |
| Chromosome | 14 |
| Deletion Start | 59,626,053 |
| Deletion End | 59,630,191 |
| Deletion Size | 4,139 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 59,626,387 | 59,626,406 | AGACAGCTCGGCAGGTAGGC |
| TUP | 59,626,408 | 59,626,431 | CCCTCTTTGCACCTGGCTGCTGCT |
| TUR | 59,626,456 | 59,626,475 | CTTCCTGGTCCTTCTTGCCC |
| TDF | 59,629,897 | 59,629,918 | GACGAGATCACAGACCAATTGC |
| TDP | 59,629,952 | 59,629,974 | GAGAAATTTGTGGGCATGTTGAC |
| TDR | 59,629,952 | 59,629,974 | GAGAAATTTGTGGGCATGTTGAC |
PCR Primers
| Primer | Sequence |
| SU | CAGAAACGTTTCTTCTGTGATG |
| SD | ACACAGGTGGCAACTCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 332 bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | 298 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
