10005 Slitrk1 Status: ES cell colonies screened / QC positives
| Gene | Slitrk1 |
| Chromosome | 14 |
| Deletion Start | 109,312,490 |
| Deletion End | 109,310,378 |
| Deletion Size | 2,113 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 109,311,932 | 109,311,950 | TCGACCTCCGTGGAAACAG |
| TUP | 109,311,911 | 109,311,929 | TGAAAACACTGCCCTATGA |
| TUR | 109,311,887 | 109,311,906 | CCAGGGATCTGCTCCAAGAC |
| TDF | 109,310,594 | 109,310,612 | TCGTCACCTCCGCCTTCAC |
| TDP | 109,310,566 | 109,310,583 | ATGCTCGTGTTTATCCTG |
| TDR | 109,310,540 | 109,310,559 | CTTCTCTTGGACCGCTTTCG |
PCR Primers
| Primer | Sequence |
| SU | TGTGCCTGGATAGTTTGTGG |
| SD | GCTCAGAGCAAAGAGCTAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 342 bp | - | + | + |
| TUF | TUR | 64 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | 316 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10005C-A6 | VGB6 | C57BL/6NTac | |
| 10005C-B1 | VGB6 | C57BL/6NTac | |
| 10005C-F6 | VGB6 | C57BL/6NTac | |
| 10005C-G3 | VGB6 | C57BL/6NTac | |
