10006   Slitrk6     Status: Cancelled

GeneSlitrk6
Chromosome14
Deletion Start110,752,270
Deletion End110,749,697
Deletion Size2,574
Genome Build38
MGI IDMGI:2443198
NCBI Gene ID239250
BAC ID68h18
BAC LibraryBAC ES release 1
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 14

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF110,751,684110,751,703CGTGGGAACCAGTTGCAAAC
TUP110,751,660110,751,679CCTTATGTTGGCTTTTTAGA
TUR110,751,639110,751,658TCCAATATCCGGCCAATGTG
TDF110,750,545110,750,563AATCCTTCCATGCCAACCC
TDP110,750,517110,750,534TGTTATTGTCACTTCTCC
TDR110,750,496110,750,514GCTGTATCTGCCGCCACTG

PCR Primers

PrimerSequence
SUGGAAGCATTCTCTTCTCTGTG
SDGAGTATCCAAGAAAACTGGAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev405 bp-++
TUFTUR65 bp++-
TDFTDR68 bp++-
NeoFwdSD381 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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