| Gene | Zzef1 |
| Chromosome | 11 |
| Deletion Start | 72,796,279 |
| Deletion End | 72,831,740 |
| Deletion Size | 35,462 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 72,796,703 | 72,796,724 | TCGCCAATAAGGTCCTGAAGAG |
| TUP | 72,796,734 | 72,796,753 | TGGCTGGCCTCCGCAAAGCA |
| TUR | 72,796,759 | 72,796,778 | AGAACCCGAGGACCCTATGG |
| TDF | 72,831,638 | 72,831,661 | CTGACCACTTGGACACACAGTATG |
| TDP | 72,831,663 | 72,831,687 | CGCCTCATCCCTCATCTCATCCATG |
| TDR | 72,831,689 | 72,831,709 | GCAGGTTCTGCCTGACAGATG |
PCR Primers
| Primer | Sequence |
| SU | GACGGATACGTTAACGGGTG |
| SD | CTCCAGCCAAGTCTCTCTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 353 bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | 322 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10007B-A4 | VGB6 | C57BL/6NTac | |
