10008   Antxr2     Status: ES cell colonies screened / QC no positives

GeneAntxr2
Chromosome5
Deletion Start
Deletion End
Deletion Size
Genome Build37
MGI IDMGI:1919164
NCBI Gene ID71914
BAC ID212H8
BAC LibrarybMQ
CassetteTM-ZEN-Ub1
Clones AvailableNo

Sequence Downloads and Strategy Information

  1. 2,000 Bases Upstream (not yet available)
  2. 2,000 Bases Downstream (not yet available)
  3. Primer Sequences
  4. Cassette
  5. Design Diagram
  6. PCR Conditions
  7. Genotyping Strategy

Primer Genomic Coordinates on Chromosome 5

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUFGACGAGATCACAGACCAATTGC
TUPCATTGTTCATGGTTCCCTCGGGCA
TURGAAATTTGTGGGCATGTTGACA
TDF98,003,26598,003,284GGACCAGGTTTTCCCTGTCA
TDP98,003,23298,003,260TGGATTTCAAGCTCTCAAAGGCATCATCA
TDR98,003,20998,003,229ATCCGGAGGCACACTTACAGA

PCR Primers

PrimerSequence
SUAAGAAGTGTGGTCTTCCTGG
SDCTGGAGAAACTCTAAAGGTAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TDFTDR76 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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