10009 Celsr3 Status: Germline transmission achieved
| Gene | Celsr3 |
| Chromosome | 9 |
| Deletion Start | 108,728,637 |
| Deletion End | 108,753,663 |
| Deletion Size | 25,027 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 108,728,988 | 108,729,007 | TGGGTAGTCGCGAGCAAGAG |
| TUP | 108,729,020 | 108,729,046 | CCAGGGTATCTGTTATGTTGGCACCCA |
| TUR | 108,729,049 | 108,729,067 | CCGCCCACAAGAGGAGATC |
| TDF | 108,753,494 | 108,753,518 | TGCTAATGAGTCTAGGGTCCTGATG |
| TDP | 108,753,523 | 108,753,547 | CAGGACTCTGAGCCCTCCCTCCACA |
| TDR | 108,753,603 | 108,753,627 | GGAGTTGAATGTAGAAGTGGAAAGC |
PCR Primers
| Primer | Sequence |
| SU | CAGAACTGCCATGCGCGAC |
| SD | TCTCTCTGGTTACAAAGGTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 460 bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 134 bp | + | + | - |
| NeoFwd | SD | 302 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10009A-G12 | VGB6 | C57BL/6NTac | |
| 10009A-F9 | VGB6 | C57BL/6NTac | |
