10010 Cd164l2 Status: ES cell colonies screened / QC no positives
| Gene | Cd164l2 |
| Chromosome | 4 |
| Deletion Start | 133,220,966 |
| Deletion End | 133,224,264 |
| Deletion Size | 3,299 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 133,222,144 | 133,222,164 | TATTGTGGATGCCCTGTCTCC |
| TUP | 133,222,166 | 133,222,192 | CAGCTTCCCATCATCATTCCACAGAGG |
| TUR | 133,222,195 | 133,222,215 | ACCTGTGGTACTTGGCTTCGG |
| TDF | 133,223,571 | 133,223,591 | TGTTGTGACCTCCACTCTGCC |
| TDP | 133,223,593 | 133,223,616 | CATTCTGCAGGGAGTCCCCCCATC |
| TDR | 133,223,618 | 133,223,637 | ACCTGGGCTGTGGTCTTCAG |
PCR Primers
| Primer | Sequence |
| SU | ACCCATCCCTTTTGTTCAGG |
| SD | GAATCATGGGGTGGAAACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 345 bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | 376 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
