10012 Ltk Status: ES cell colonies screened / QC no positives
| Gene | Ltk |
| Chromosome | 2 |
| Deletion Start | 119,760,291 |
| Deletion End | 119,751,573 |
| Deletion Size | 8,719 |
| Genome Build | 38 |
|
| MGI ID | MGI:96840 |
| NCBI Gene ID | 17005 |
| BAC ID | 248a10 |
| BAC Library | BAC ES release 2 |
| Cassette | ZEN-Ub1 |
| Clones Available | No
|
|
Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 119,759,627 | 119,759,646 | TTTTGCCGGTGCTGGTACTC |
| TUP | 119,759,599 | 119,759,623 | TCCCAGGAGCAGAAAGTCACCCCCA |
| TUR | 119,759,577 | 119,759,596 | GCTGGCTCCAATTTACTGGG |
| TDF | 119,751,840 | 119,751,859 | GGAAACAGGTCACTGGAGGG |
| TDP | 119,751,807 | 119,751,832 | TCCCCAAAGCCCCTAGAGCTGAGTTC |
| TDR | 119,751,785 | 119,751,805 | TCCCCAGCTCTTCAAGTTCTG |
PCR Primers
| Primer | Sequence |
| SU | TGCCATAGGTGGGGACTAG |
| SD | AGCCCTGGAATGAGCAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 360 bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | 298 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
