10014   Srpx     Status: Cancelled

GeneSrpx
ChromosomeX
Deletion Start
Deletion End
Deletion Size
Genome Build37
MGI IDMGI:1858306
NCBI Gene ID51795
BAC ID474n1
BAC LibraryBAC ES release 2
CassetteTM-ZEN-Ub1
Clones AvailableNo

Sequence Downloads and Strategy Information

  1. 2,000 Bases Upstream (not yet available)
  2. 2,000 Bases Downstream (not yet available)
  3. Primer Sequences
  4. Cassette
  5. Design Diagram
  6. PCR Conditions
  7. Genotyping Strategy

Primer Genomic Coordinates on Chromosome X

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF133,911,376133,911,396CTTTCTCTCTTCCTCCCAGGG
TUP133,911,406133,911,433AGGATTTGTATAGCCGCTTTTTTGGCCA
TUR133,911,435133,911,455CTGTATTGTGATTGACCCCCG
TDF133,926,253133,926,273GATCCGTTGTCCTCACTCTCG
TDP133,926,276133,926,307AGAAAATGGCAGAGCCAGAGAAACTAACTGCT
TDR133,926,318133,926,338CTTTCACTAAGGGTGGGTCCC

PCR Primers

PrimerSequence
SUGAGCCAGGTTCACTCTGAG
SDGAAAACATGTTTCACACTGAGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR80 bp++-
TDFTDR86 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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