10015 Iglon5 Status: Cancelled
| Gene | Iglon5 |
| Chromosome | 7 |
| Deletion Start | 43,480,635 |
| Deletion End | 43,474,408 |
| Deletion Size | 6,228 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,480,523 | 43,480,543 | TCACAAGGCTACTCCCCTTCC |
| TUP | 43,480,494 | 43,480,521 | CCAACGTACTCTGCCTGTGAAAGTCCCA |
| TUR | 43,480,469 | 43,480,489 | TGGCTCCAGATGAACATGATG |
| TDF | 43,473,338 | 43,473,357 | TTCACCTGCCCCAGAGTCTG |
| TDP | 43,473,310 | 43,473,333 | TAGCAGCAGTTTCCCCGGAGTGGG |
| TDR | 43,473,282 | 43,473,302 | TGTCAGCTTCCTGTGTGGATG |
PCR Primers
| Primer | Sequence |
| SU | TTGGGTGTCATCTCTGGAAG |
| SD | CAGCCTGAGCTAAGAGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 481 bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | 326 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
