| Gene | Dyrk1b |
| Chromosome | 7 |
| Deletion Start | 28,181,595 |
| Deletion End | 28,187,003 |
| Deletion Size | 5,409 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 28,182,533 | 28,182,552 | CTTGCCCGGGTCACTTAAGG |
| TUP | 28,182,556 | 28,182,577 | CGGTTTCCTGGCTGCCTGAGCA |
| TUR | 28,182,584 | 28,182,603 | GCCGCTTCTTCTTCGCATAG |
| TDF | 28,186,227 | 28,186,250 | CTAGATTTCAAACTTGGGCTGTTG |
| TDP | 28,186,252 | 28,186,278 | CAGCCGTCATTCACACTTGCTCTGGTC |
| TDR | 28,186,285 | 28,186,304 | TCCTCCACCCCAAGCTTAGG |
PCR Primers
| Primer | Sequence |
| SU | CTGTTTGTACATGGCTACCTG |
| SD | GGAGGAGCAAGGCCATCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 370 bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | 341 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10019E-B6 | VGB6 | C57BL/6NTac | |
| 10019E-D2 | VGB6 | C57BL/6NTac | |
| 10019E-D5 | VGB6 | C57BL/6NTac | |
| 10019E-D9 | VGB6 | C57BL/6NTac | |
| 10019E-G7 | VGB6 | C57BL/6NTac | |
| 10019E-C9 | VGB6 | C57BL/6NTac | |
