10021 Fgf11 Status: ES cell clone microinjected
| Gene | Fgf11 |
| Chromosome | 11 |
| Deletion Start | 69,615,124 |
| Deletion End | 69,611,680 |
| Deletion Size | 3,445 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,614,176 | 69,614,196 | CCCACCAGGAGAGGAGAGATG |
| TUP | 69,614,149 | 69,614,173 | TGGACGGGAATCACATACCAGCCCT |
| TUR | 69,614,121 | 69,614,141 | GCCAG AGGGACTCCCTAACTG |
| TDF | 69,612,124 | 69,612,142 | GGAAGGCCGGGTCATGAAG |
| TDP | 69,612,093 | 69,612,120 | CAGCTGCCTTGGTCTTCTTGACTCGGTT |
| TDR | 69,612,071 | 69,612,090 | AGGAGCTTGGGCACAAAGTG |
PCR Primers
| Primer | Sequence |
| SU | TGGTACAGAGGCCAGAGAG |
| SD | GGATCTAAGAGCTCAGCAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TCATTCTCAGTATTGTTTTGCC |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 409 bp | - | + | + |
| TUF | TUR | 56 bp | + | + | - |
| TDF | TDR | 54 bp | + | + | - |
| NeoFwd | SD | 282 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10021A-C12 | VGB6 | C57BL/6NTac | |
| 10021A-H7 | VGB6 | C57BL/6NTac | |
