10022 Fjx1 Status: Germline transmission achieved
| Gene | Fjx1 |
| Chromosome | 2 |
| Deletion Start | 102,291,742 |
| Deletion End | 102,290,364 |
| Deletion Size | 1,379 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 102,290,879 | 102,290,900 | GCTGGTGGACTTGGTACAATGG |
| TUP | 102,290,854 | 102,290,872 | CTGATCCTCTTCGATTACC |
| TUR | 102,290,827 | 102,290,846 | TCACAAGCCGGTCAAAGTTG |
| TDF | 102,290,568 | 102,290,586 | GGTTGCTGCGCCTCTACAG |
| TDP | 102,290,540 | 102,290,561 | CGAACCGCGTTTCCCAGAGCTG |
| TDR | 102,290,477 | 102,290,497 | GTGTTTGGCGAGGAAGTCAAG |
PCR Primers
| Primer | Sequence |
| SU | TCCGGGCGCCTGTGAATC |
| SD | GCAGCCAGTTTCTTCGGATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 375 bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | 328 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10022B-C9 | VGB6 | C57BL/6NTac | |
| 10022B-D8 | VGB6 | C57BL/6NTac | |
| 10022B-E11 | VGB6 | C57BL/6NTac | |
| 10022B-F11 | VGB6 | C57BL/6NTac | |
| 10022B-H3 | VGB6 | C57BL/6NTac | |
| 10022B-C8 | VGB6 | C57BL/6NTac | |
