10027 Angpt4 Status: ES cell colonies screened / QC no positives
| Gene | Angpt4 |
| Chromosome | 2 |
| Deletion Start | 151,911,317 |
| Deletion End | 151,944,655 |
| Deletion Size | 33,339 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 151,911,677 | 151,911,697 | GGGTCGTGTGATGGGCTAGAG |
| TUP | 151,911,755 | 151,911,782 | ACCACATAGAGCCTGGGCCTCTCTGTCT |
| TUR | 151,911,790 | 151,911,809 | CTGGACCCCACATCCCAAAG |
| TDF | 151,944,379 | 151,944,399 | CCACCACCTTCAGCAAATTGG |
| TDP | 151,944,401 | 151,944,427 | ACCCCAAAATGACAGTCCATGAGCCTT |
| TDR | 151,944,435 | 151,944,454 | GATTGGTGGCCGAGATTCAG |
PCR Primers
| Primer | Sequence |
| SU | AGTGCATGGGCGGCCTTGAG |
| SD | TGCGGACATCTCAAGCCGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 436 bp | - | + | + |
| TUF | TUR | 133 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | 493 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
