| Gene | Il20 |
| Chromosome | 1 |
| Deletion Start | 130,911,223 |
| Deletion End | 130,907,395 |
| Deletion Size | 3,829 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 130,911,175 | 130,911,194 | CTCCGCTGTGGGTTTTCTTC |
| TUP | 130,911,145 | 130,911,172 | TGGACTCCTTTAACTGGGCTCAAGACCC |
| TUR | 130,911,124 | 130,911,143 | TCACACAGCTTCCCAAATGG |
| TDF | 130,907,956 | 130,907,976 | CCTCCCTGAATCAGCCTAGGT |
| TDP | 130,907,938 | 130,907,954 | ATCACCTCCAGGAAGGA |
| TDR | 130,907,916 | 130,907,935 | AACTCTGGGAGCAGCATCCA |
PCR Primers
| Primer | Sequence |
| SU | TGCGGAACTGTGAAGGAGAC |
| SD | AATATACACATAGCTAGCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 392 bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 61 bp | + | + | - |
| NeoFwd | SD | 260 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10036A-C9 | VGB6 | C57BL/6NTac | |
| 10036B-A4 | VGB6 | C57BL/6NTac | |
| 10036B-A6 | VGB6 | C57BL/6NTac | |
| 10036B-C3 | VGB6 | C57BL/6NTac | |
| 10036B-E3 | VGB6 | C57BL/6NTac | |
| 10036B-G3 | VGB6 | C57BL/6NTac | |
