Information regarding availability, timelines and delivery of materials can be found at www.komp.org or www.knockoutmouse.org.

10038 Il1r2 Status: ES cell colonies screened / QC positives

Gene	Il1r2
Chromosome	1
Deletion Start	40,159,258
Deletion End	40,182,052
Deletion Size	22,795
Genome Build	37

MGI ID	MGI:96546
NCBI Gene ID	16178
BAC ID	456A19
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	Yes

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome 1

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	40,181,894	40,181,913	ACGTTCTCCTGGAGCATTGC
TUP	40,181,916	40,181,945	TGGCACCTCTGTCTCTGATCATCTTGGTTG
TUR	40,181,952	40,181,971	CCGTCTGCGCATCCATATTG
TDF	40,181,894	40,181,913	ACGTTCTCCTGGAGCATTGC
TDP	40,181,916	40,181,945	TGGCACCTCTGTCTCTGATCATCTTGGTTG
TDR	40,181,952	40,181,971	CCGTCTGCGCATCCATATTG

PCR Primers

Primer	Sequence
SU	TCAAACATTCTCGTTCTCAGG
SD	AAGGATGAGGAGGGACGTGAC
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TCATTCTCAGTATTGTTTTGCC
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	284 bp	-	+	+
TUF	TUR	59 bp	+	+	-
TDF	TDR	59 bp	+	+	-
NeoFwd	SD	407 bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+

Available Clones

ES Cell ordering information can be found at the KOMP Repository.

Clone Name	Parental ES Cell	Mouse Strain	Cassette Copy Number
10038G-D3	VGB6	C57BL/6NTac