10039 Il3ra Status: ES cell colonies screened / QC no positives
| Gene | Il3ra |
| Chromosome | 14 |
| Deletion Start | 14,347,114 |
| Deletion End | 14,355,426 |
| Deletion Size | 8,313 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 14,347,953 | 14,347,973 | CCATCCAGAACCTGCATATCG |
| TUP | 14,347,978 | 14,348,002 | CGCCCACTACACACTGAGCTGGGAC |
| TUR | 14,348,059 | 14,348,077 | GCCCCTCACCCAGACAAAG |
| TDF | 14,354,948 | 14,354,967 | CCCAACCCAGGTGACTGATG |
| TDP | 14,354,970 | 14,354,996 | TCTACCCCTGTCCCTGCCCCTCTCTCT |
| TDR | 14,355,007 | 14,355,026 | GGCGGTAGAGCAGCGACTTC |
PCR Primers
| Primer | Sequence |
| SU | TCACCGCTGCCATCCCTCTG |
| SD | TCTGTCTCAGCTCCTGTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 423 bp | - | + | + |
| TUF | TUR | 125 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | 365 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
