10040 Il10ra Status: ES cell colonies screened / QC no positives
| Gene | Il10ra |
| Chromosome | 9 |
| Deletion Start | 45,269,069 |
| Deletion End | 45,255,506 |
| Deletion Size | 13,564 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 45,268,635 | 45,268,654 | CACCCCATCCCTTCTTTCTG |
| TUP | 45,268,609 | 45,268,633 | CCACCCTTCCCGACCACCCTGTTAG |
| TUR | 45,268,580 | 45,268,599 | GGCAGCGGAGATGAATTGTG |
| TDF | 45,256,436 | 45,256,455 | GCCTCTCCTCCCAGGTCTTC |
| TDP | 45,256,402 | 45,256,426 | CACGACTTCTTCCCAGCCAACCCTC |
| TDR | 45,256,376 | 45,256,395 | AATGGCATCGGGAGTTTCTG |
PCR Primers
| Primer | Sequence |
| SU | AGAGTTAAGAGCCCAGCCAC |
| SD | TGAGGCATGAGGAGGTCTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 380 bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | 333 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
