| Gene | Il13ra1 |
| Chromosome | X |
| Deletion Start | 36,126,635 |
| Deletion End | 36,155,629 |
| Deletion Size | 28,995 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,127,585 | 36,127,607 | CAAGGGTAGCTTAGGCAGAATCC |
| TUP | 36,127,611 | 36,127,643 | TGCTCCCAAGTTGCTAAATAAGATCAAGTCTGC |
| TUR | 36,127,650 | 36,127,672 | GCTTCTCTGTGAAAATGGCAAAC |
| TDF | 36,154,376 | 36,154,396 | GGAGAGGAAAGGGCTGAAGAC |
| TDP | 36,154,398 | 36,154,421 | TGGCTAGGCAGCAGCCCACTTGTC |
| TDR | 36,154,425 | 36,154,444 | CAACCCTAGCGATGCACATG |
PCR Primers
| Primer | Sequence |
| SU | CACCCAAATAGTCATCACAG |
| SD | GCAAATATTGTGTGATTCCA |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 474 bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | 401 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10041A-F10 | VGB6 | C57BL/6NTac | |
| 10041B-D12 | VGB6 | C57BL/6NTac | |
