10042 Nov Status: ES cell colonies screened / QC no positives
| Gene | Nov |
| Chromosome | 15 |
| Deletion Start | 54,745,956 |
| Deletion End | 54,752,506 |
| Deletion Size | 6,551 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 54,746,325 | 54,746,344 | CCCTGTGACCAGAGCAGTGG |
| TUP | 54,746,346 | 54,746,370 | CTCTACTGTGACCGCAGCGCAGACC |
| TUR | 54,746,375 | 54,746,394 | TGCAAATGCCAGTCTGGTTG |
| TDF | 54,752,401 | 54,752,421 | CCAGTCATGGTCATTGGAACC |
| TDP | 54,752,426 | 54,752,452 | CCTGCTACTCCAACTGCCCTCAGAACA |
| TDR | 54,752,458 | 54,752,478 | CTCCAGATCTTGGAGGAAGGC |
PCR Primers
| Primer | Sequence |
| SU | ACACAGAGGCATACTCAGGC |
| SD | AACTAGATCATTTATGTGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 376 bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | 357 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
