10043 Epha1 Status: Germline transmission achieved
| Gene | Epha1 |
| Chromosome | 6 |
| Deletion Start | 42,323,206 |
| Deletion End | 42,310,556 |
| Deletion Size | 12,651 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 42,322,847 | 42,322,868 | GCATTCCTACTGCAGCATCATG |
| TUP | 42,322,813 | 42,322,835 | ATGCCCACCCCTGACTTTGCAGC |
| TUR | 42,322,784 | 42,322,804 | AGGCTTTCCGCCTCAGACTTC |
| TDF | 42,311,116 | 42,311,134 | GATGGACAGCCCCAGAAGC |
| TDP | 42,311,089 | 42,311,112 | TGCCCATCGGATCTTCACCACAGC |
| TDR | 42,311,066 | 42,311,086 | TCCCAAAGCTCCACACATCAC |
PCR Primers
| Primer | Sequence |
| SU | TTCCTAGCAACACCCACTAG |
| SD | TGCTTCTTACCTTTCATCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 371 bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | 309 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10043A-D7 | VGB6 | C57BL/6NTac | |
| 10043A-E6 | VGB6 | C57BL/6NTac | |
| 10043A-F7 | VGB6 | C57BL/6NTac | |
| 10043B-G12 | VGB6 | C57BL/6NTac | |
| 10043B-G11 | VGB6 | C57BL/6NTac | |
