10045 Grem2 Status: ES cell colonies screened / QC no positives
| Gene | Grem2 |
| Chromosome | 1 |
| Deletion Start | 174,837,278 |
| Deletion End | 174,836,755 |
| Deletion Size | 524 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 174,836,974 | 174,836,992 | GCTACGGCCAGTGCAACTC |
| TUP | 174,836,945 | 174,836,968 | ACATCCCGCGACACGTGAAGAAGG |
| TUR | 174,836,913 | 174,836,931 | GCAGAAAGCGCAGGATTGG |
| TDF | 174,836,974 | 174,836,993 | TGCTACGGCCAGTGCAACTC |
| TDP | 174,836,944 | 174,836,969 | TCCTTCTTCACGTGTCGCGGGATGTA |
| TDR | 174,836,913 | 174,836,931 | GCAGAAAGCGCAGGATTGG |
PCR Primers
| Primer | Sequence |
| SU | CAAAACACAGCACGGCTTTC |
| SD | AGAGACACAAGTGTAGCCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 300 bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | 293 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
