| Gene | Vnn3 |
| Chromosome | 10 |
| Deletion Start | |
| Deletion End | |
| Deletion Size | |
| Genome Build | 37 |
|
|
Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 23,851,895 | 23,851,915 | TGTTTTGAGCAGGGTGCACAT |
| TUP | 23,851,917 | 23,851,946 | CATAGATTCCATCTTCTGGCGTCACAATGA |
| TUR | 23,851,949 | 23,851,969 | GGTCTCCCTGGTGAAGATCCA |
| TDF | | | CGGGATGTTGTCCTCTGCTATC |
| TDP | | | CCATTGGCTTGGTGGGT |
| TDR | | | GTTCCTGGAGGTAACCGTGAAG |
PCR Primers
| Primer | Sequence |
| SU | ATGGGAATATTGGTGTGCTAG |
| SD | GTTTCCTCTCACTTGCTTTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
