10047 Bok Status: ES cell colonies screened / QC positives
| Gene | Bok |
| Chromosome | 1 |
| Deletion Start | 93,686,430 |
| Deletion End | 93,695,193 |
| Deletion Size | 8,764 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 93,686,472 | 93,686,491 | TCTTTGCAGCGGAGATCATG |
| TUP | 93,686,512 | 93,686,535 | TGGGCCACCAGCTCCTTGTCTGTG |
| TUR | 93,686,538 | 93,686,557 | CTCCCGGCCTAGTGCCTTAG |
| TDF | 93,694,998 | 93,695,017 | GGGTGTCTGGCGTTGCTAAC |
| TDP | 93,695,033 | 93,695,059 | ACCACACACTTGAGGACATCCGTCTGG |
| TDR | 93,695,065 | 93,695,083 | GAGCGGAAGCCAGGATCTG |
PCR Primers
| Primer | Sequence |
| SU | TTTAGAACCTAAAAAGAACC |
| SD | TACTCTGCATCCCTGGCTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 378 bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | 472 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
