10053 Kcne3 Status: Germline transmission achieved
| Gene | Kcne3 |
| Chromosome | 7 |
| Deletion Start | 107,332,668 |
| Deletion End | 107,333,002 |
| Deletion Size | 335 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 107,331,198 | 107,331,219 | GCTCTGGGCCTTTCCTGTTAAC |
| TUP | 107,331,230 | 107,331,253 | TGGCTGCTTCCAGATGCACCTTGC |
| TUR | 107,331,257 | 107,331,277 | TGTCCCACAACCCCTCAGTTC |
| TDF | 107,332,702 | 107,332,723 | CGGGACTGAGACCTGGTACATG |
| TDP | 107,332,726 | 107,332,750 | CCTCCATGCTGTGCTGAAGGCTCTG |
| TDR | 107,332,760 | 107,332,781 | GGCAGAGCAAGTGACTGTGAAG |
PCR Primers
| Primer | Sequence |
| SU | ACGCACTGTGGGATGCTTC |
| SD | ATGTGTTCATGGGCTCTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TCATTCTCAGTATTGTTTTGCC |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 355 bp | - | + | + |
| TUF | TUR | 60 bp | + | + | - |
| TDF | TDR | 59 bp | + | + | - |
| NeoFwd | SD | 317 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
