10055 Bambi Status: Germline transmission achieved
| Gene | Bambi |
| Chromosome | 18 |
| Deletion Start | 3,508,192 |
| Deletion End | 3,512,398 |
| Deletion Size | 4,207 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 3,508,486 | 3,508,504 | CGCCGTTGGGCTCAAGTAC |
| TUP | 3,508,506 | 3,508,528 | TTTTCTCCGGACAGTCCCGTCGG |
| TUR | 3,508,531 | 3,508,549 | CCGGTCCAGTTGGGATCTG |
| TDF | 3,512,062 | 3,512,081 | GTTCCGCGCAGCTGTGATAG |
| TDP | 3,512,092 | 3,512,115 | AGCACCAAGATCAGTCCGCCAGCA |
| TDR | 3,512,117 | 3,512,137 | TCGCAAGGCCAACATAATGAG |
PCR Primers
| Primer | Sequence |
| SU | TATTTACGGCGGGGACTCTG |
| SD | TTACTTCAGTCAGCTGCAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TCATTCTCAGTATTGTTTTGCC |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 401 bp | - | + | + |
| TUF | TUR | 46 bp | + | + | - |
| TDF | TDR | 56 bp | + | + | - |
| NeoFwd | SD | 359 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10055A-D10 | VGB6 | C57BL/6NTac | |
| 10055A-G8 | VGB6 | C57BL/6NTac | |
| 10055A-H10 | VGB6 | C57BL/6NTac | |
| 10055B-B5 | VGB6 | C57BL/6NTac | |
| 10055B-B6 | VGB6 | C57BL/6NTac | |
| 10055B-C1 | VGB6 | C57BL/6NTac | |
