| Gene | Caly |
| Chromosome | 7 |
| Deletion Start | 140,074,072 |
| Deletion End | 140,070,327 |
| Deletion Size | 3,746 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 140,073,986 | 140,074,006 | ATGGACAGCGTCCCTCTGATC |
| TUP | 140,073,956 | 140,073,979 | CTGGATGTCAGCCAGCTTCAGCCG |
| TUR | 140,073,932 | 140,073,954 | CACACTCACCTGGTCTGAGAATG |
| TDF | 140,070,494 | 140,070,513 | CCGCCAAGGAGGAGCATAAG |
| TDP | 140,070,408 | 140,070,433 | TCCAAAGACTTCCCTGACGGCTCGGT |
| TDR | 140,070,378 | 140,070,398 | GCCTTCTGCGGGTCTTCTTTC |
PCR Primers
| Primer | Sequence |
| SU | AAAACACATGGCAGAGCAGG |
| SD | AGGCAGTGTGCATGTCAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 393 bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 136 bp | + | + | - |
| NeoFwd | SD | 430 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10056A-H11 | VGB6 | C57BL/6NTac | |
| 10056A-C12 | VGB6 | C57BL/6NTac | |
| 10056A-E2 | VGB6 | C57BL/6NTac | |
| 10056A-E7 | VGB6 | C57BL/6NTac | |
| 10056A-E8 | VGB6 | C57BL/6NTac | |
