10058 Cadm3 Status: Cancelled
| Gene | Cadm3 |
| Chromosome | 1 |
| Deletion Start | 173,367,491 |
| Deletion End | 173,336,972 |
| Deletion Size | 30,520 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 173,367,208 | 173,367,226 | GGCGTGTGCACCTCCTATG |
| TUP | 173,367,160 | 173,367,184 | AGATCCACATCCCTGCTGCACACGT |
| TUR | 173,367,128 | 173,367,148 | CCCCGGTTGCTCAAAATAGAC |
| TDF | 173,337,281 | 173,337,301 | GGGATGTTGGCAACAAAATGC |
| TDP | 173,337,233 | 173,337,262 | CATCTTTCTCTTGCCTGGAGCTTTCTTCCA |
| TDR | 173,337,211 | 173,337,230 | CCCACCCACTCACACCATTG |
PCR Primers
| Primer | Sequence |
| SU | ATCCCGGCGACTGCAGCAGC |
| SD | ATTACAGATTCGTTGCTCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 293 bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | 390 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
