| Gene | Tmem98 |
| Chromosome | 11 |
| Deletion Start | 80,812,562 |
| Deletion End | 80,821,419 |
| Deletion Size | 8,876 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 80,812,568 | 80,812,587 | GTGGTGATCGTCGCCATAGG |
| TUP | 80,812,592 | 80,812,615 | CTGGCCACCATTTTCCTGGCCTCA |
| TUR | 80,812,622 | 80,812,642 | CCTACAGACCACCACCAAAGC |
| TDF | 80,821,281 | 80,821,299 | GGGCCTGGACTGGATTGAC |
| TDP | 80,821,303 | 80,821,326 | TCACTGTCTGCCGCTGAGGAGCAC |
| TDR | 80,821,331 | 80,821,350 | GGCTGCCTCTCGAAGGACTT |
PCR Primers
| Primer | Sequence |
| SU | TTGCAGGGGGATGTGAGGGAGC |
| SD | TGTAGCAGCGAGCCCGGCAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 321 bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | 320 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10059A-B3 | VGB6 | C57BL/6NTac | |
| 10059A-D10 | VGB6 | C57BL/6NTac | |
| 10059A-E10 | VGB6 | C57BL/6NTac | |
| 10059A-F8 | VGB6 | C57BL/6NTac | |
| 10059A-H6 | VGB6 | C57BL/6NTac | |
