10060 Amigo2 Status: Cancelled
| Gene | Amigo2 |
| Chromosome | 15 |
| Deletion Start | 97,246,536 |
| Deletion End | 97,244,959 |
| Deletion Size | 1,578 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 97,246,447 | 97,246,467 | CTGTTGGTGATCGCAGTGATG |
| TUP | 97,246,410 | 97,246,433 | CCTCAGGAATGTGCCCCACTGCTT |
| TUR | 97,246,389 | 97,246,408 | CAATGTCGGTGGCACAGATG |
| TDF | 97,245,309 | 97,245,329 | CCTGCGTGGCCAGTATAGTTC |
| TDP | 97,245,275 | 97,245,301 | ACTGTATCTGTACCTGACGCCGTGCCC |
| TDR | 97,245,252 | 97,245,272 | GTCTCTTGGCTTTGCATTTGC |
PCR Primers
| Primer | Sequence |
| SU | TTTCTCATGCTGAGACTGTC |
| SD | AGGCTAAGATGCTGTTACTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 404 bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | 312 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
