10061 Il1rl2 Status: ES cell colonies screened / QC no positives
| Gene | Il1rl2 |
| Chromosome | 1 |
| Deletion Start | 40,327,364 |
| Deletion End | 40,356,964 |
| Deletion Size | 29,601 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 40,327,934 | 40,327,954 | GCCCAGACCAGCTTACAGAGA |
| TUP | 40,327,956 | 40,327,977 | CAATCTCATTGATAAAACACCT |
| TUR | 40,327,982 | 40,328,003 | CCATTAGCCATCTTCGGAAATG |
| TDF | 40,356,398 | 40,356,416 | TGGGCAGAAGGCCAATGAC |
| TDP | 40,356,430 | 40,356,455 | AACAGGAGTCCCTCTGCTCAGCTCCG |
| TDR | 40,356,461 | 40,356,481 | GGGTTTCAATCCCACCATACG |
PCR Primers
| Primer | Sequence |
| SU | TGGTTCTGTGGAGGGTCCTG |
| SD | GGTCACTACCTGTGTTCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 465 bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | 296 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
