10062 Adam33 Status: ES cell colonies screened / QC positives
| Gene | Adam33 |
| Chromosome | 2 |
| Deletion Start | 130,889,247 |
| Deletion End | 130,877,006 |
| Deletion Size | 12,242 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 130,888,629 | 130,888,648 | CGGGACGACAGGGCTCTTAG |
| TUP | 130,888,594 | 130,888,622 | CAAGCCTGTAGGGTGAATCTCCAAACTCC |
| TUR | 130,888,572 | 130,888,592 | GCCAGCCTAGTGCTTGCTATG |
| TDF | 130,877,586 | 130,877,604 | GGCAGAGCACGGCTAAACG |
| TDP | 130,877,551 | 130,877,576 | AAGGCACAGTTCCCACCAAGGGAGAC |
| TDR | 130,877,523 | 130,877,543 | GGCCCACCTAGAGCTGAAGTG |
PCR Primers
| Primer | Sequence |
| SU | AGAGAGGTTCTCTAAGAACC |
| SD | AGAAGAACCACAGTGTCTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 378 bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | 333 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10062A-F10 | VGB6 | C57BL/6NTac | |
| 10062B-B10 | VGB6 | C57BL/6NTac | |
