10064   Calcb     Status: Germline transmission achieved

GeneCalcb
Chromosome7
Deletion Start121,863,240
Deletion End121,865,651
Deletion Size2,412
Genome Build37
MGI IDMGI:2151254
NCBI Gene ID116903
BAC ID221G22
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 7

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF121,863,662121,863,684TGCAGGACTATGAGCAGATGAAG
TUP121,863,687121,863,713TCTTGCTCCTCCTGTTCCAGCTTCCTG
TUR121,863,716121,863,737GAGACTTACCGGGAGCCCTTAG
TDF121,864,831121,864,851TCTGTTGCGTGTGCATTTCTC
TDP121,864,858121,864,884CCGACACACTAAAGCTGCTGTTGCCAG
TDR121,864,886121,864,905CCACTCTGGGCCACTGATTC

PCR Primers

PrimerSequence
SUTTTCTTCACAGATGAGAG
SDAGCTGGGCCCATGTGTGAGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTCATTCTCAGTATTGTTTTGCC
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev384 bp-++
TUFTUR55 bp++-
TDFTDR56 bp++-
NeoFwdSD396 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
10064A-A10VGB6C57BL/6NTac
10064A-B12VGB6C57BL/6NTac
10064A-D11VGB6C57BL/6NTac
10064A-F2VGB6C57BL/6NTac
10064A-H3VGB6C57BL/6NTac
10064A-H7VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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