10065 Scgb3a2 Status: ES cell clone microinjected
| Gene | Scgb3a2 |
| Chromosome | 18 |
| Deletion Start | 43,764,402 |
| Deletion End | 43,767,068 |
| Deletion Size | 2,667 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,766,114 | 43,766,137 | TTAGTTCATTCAGGCTTGGTCTTG |
| TUP | 43,766,140 | 43,766,166 | AAGGAGACTTGCCATAAAGCTTCGCCA |
| TUR | 43,766,170 | 43,766,192 | TTGTCCACAGTGATAGCCTTCAG |
| TDF | 43,766,114 | 43,766,137 | TTAGTTCATTCAGGCTTGGTCTTG |
| TDP | 43,766,140 | 43,766,166 | AAGGAGACTTGCCATAAAGCTTCGCCA |
| TDR | 43,766,170 | 43,766,192 | TTGTCCACAGTGATAGCCTTCAG |
PCR Primers
| Primer | Sequence |
| SU | TGTGATGAAAAGTGGAAAAC |
| SD | CAGTTTACAAATGATAGAATGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 353 bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | 357 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
