10068 Slamf7 Status: ES cell colonies screened / QC no positives
| Gene | Slamf7 |
| Chromosome | 1 |
| Deletion Start | 171,648,924 |
| Deletion End | 171,634,840 |
| Deletion Size | 14,085 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 171,648,702 | 171,648,724 | TGGGTTTCAGGAGATCTGTGTTC |
| TUP | 171,648,649 | 171,648,678 | AAGTCCAGGGAAGCATTCACATTCTCCACT |
| TUR | 171,648,615 | 171,648,636 | AAAGGAGGGAAAGCGAAAAGAC |
| TDF | 171,635,865 | 171,635,885 | AACCTGAGGAGGGAGATTTGC |
| TDP | 171,635,834 | 171,635,863 | TGCTGTAGAACATCTGCTTTGTAGCCTCCC |
| TDR | 171,635,812 | 171,635,832 | TCACCACATCTGCGGCTTAAG |
PCR Primers
| Primer | Sequence |
| SU | CCTACAAAGTGTGTGTGATTC |
| SD | CTGTCTATGAGCACAGCCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 336 bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | 307 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
