| Gene | Sost |
| Chromosome | 11 |
| Deletion Start | 101,966,970 |
| Deletion End | 101,963,797 |
| Deletion Size | 3,174 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 101,966,918 | 101,966,936 | TTGTGCACGCTGCCTTCTG |
| TUP | 101,966,874 | 101,966,898 | TGGCATCATTCCTGAAGGCTTGCCA |
| TUR | 101,966,850 | 101,966,868 | CTCCAAGCCCTGGGATGAC |
| TDF | 101,964,405 | 101,964,424 | AGAAGCCAGGGTGGGATCTC |
| TDP | 101,964,379 | 101,964,402 | TGCTCCCCGGTGCCTCCTTCCTAT |
| TDR | 101,964,355 | 101,964,375 | TGCGCTTTCGAATCTGTATGG |
PCR Primers
| Primer | Sequence |
| SU | CTGGGAGGTGCCAGCAGCA |
| SD | AGGTCTTTCGTTCCACACTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 369 bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | 360 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10069A-G10 | VGB6 | C57BL/6NTac | |
| 10069B-C7 | VGB6 | C57BL/6NTac | |
| 10069B-C10 | VGB6 | C57BL/6NTac | |
| 10069B-E8 | VGB6 | C57BL/6NTac | |
