10071 4930578C19Rik Status: Cancelled
| Gene | 4930578C19Rik |
| Chromosome | X |
| Deletion Start | 18,461,259 |
| Deletion End | 18,423,533 |
| Deletion Size | 37,727 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 18,461,139 | 18,461,160 | CCAGCTTCTCTCCCTCCTTCTC |
| TUP | 18,461,118 | 18,461,135 | TACCCAGAGTCAGAAGCA |
| TUR | 18,461,089 | 18,461,111 | ACCGAGGAATGTCTTTCCCATAG |
| TDF | 18,423,678 | 18,423,696 | CGCTGCCCTTCACAAAGAC |
| TDP | 18,423,651 | 18,423,674 | CATAGCGTCGAACCACACGGTCCA |
| TDR | 18,423,628 | 18,423,648 | GCTGCCAGCATCGACTACTTC |
PCR Primers
| Primer | Sequence |
| SU | CCAAGCAGAGGAAGAGTGAC |
| SD | GCCTCAGCTTGTTAAGTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 358 bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | 317 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
