10072 Antxr1 Status: ES cell colonies screened / QC positives
| Gene | Antxr1 |
| Chromosome | 6 |
| Deletion Start | 87,262,372 |
| Deletion End | 87,232,670 |
| Deletion Size | 29,703 |
| Genome Build | 37 |
|
|
Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 87,262,326 | 87,262,346 | CGTGGAGCAGTTGGCTCATAG |
| TUP | 87,262,294 | 87,262,315 | AAGAGACCGCGGCGCACACTCA |
| TUR | 87,262,260 | 87,262,278 | GCAGGTGGGCTGGATTCAC |
| TDF | 87,233,217 | 87,233,236 | TGAGCCCAACAAAGCAATGG |
| TDP | 87,233,192 | 87,233,215 | CCTTTCACACCAGCCAAGGGCAGG |
| TDR | 87,233,161 | 87,233,180 | CTGTTGCGCCAGGTGATGAG |
PCR Primers
| Primer | Sequence |
| SU | ATTGTCTGCTTCACTGTCAG |
| SD | AGACTCTGAGAACAGAAGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 451 bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | 350 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10072D-B6 | VGB6 | C57BL/6NTac | |
| 10072H-B7 | VGB6 | C57BL/6NTac | |
| 10072H-D2 | VGB6 | C57BL/6NTac | |
| 10072H-D10 | VGB6 | C57BL/6NTac | |
| 10072H-G1 | VGB6 | C57BL/6NTac | |
| 10072I-F4 | VGB6 | C57BL/6NTac | |
| 10072I-E2 | VGB6 | C57BL/6NTac | |
