10074 Havcr1 Status: ES cell colonies screened / QC no positives
| Gene | Havcr1 |
| Chromosome | 11 |
| Deletion Start | 46,751,160 |
| Deletion End | 46,778,613 |
| Deletion Size | 27,454 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 46,751,733 | 46,751,754 | TGTCTGGAGTGAGCAAGTTTGG |
| TUP | 46,751,762 | 46,751,789 | CTTTTCCAAGAAGGCTGAACCCTCTGCT |
| TUR | 46,778,565 | 46,778,585 | TTGTCTTCAGCTCGGGAATGC |
| TDF | 46,778,514 | 46,778,535 | GGTTGCCTTCCGTGTCTCTAAG |
| TDP | 46,778,538 | 46,778,562 | AACCGCTGCGTTCTGCAAAGCTTCA |
| TDR | 46,751,796 | 46,751,818 | GAAGATGCTAAGCCTTCGTTGAC |
PCR Primers
| Primer | Sequence |
| SU | TTGCTGCCTTATTTGTGTCC |
| SD | AATGATTACATTGACTAAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 324 bp | - | + | + |
| TUF | TUR | 26853 bp | + | + | - |
| TDF | TDR | 26740 bp | + | + | - |
| NeoFwd | SD | 370 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
