10075 Stab1 Status: ES cell colonies screened / QC no positives
| Gene | Stab1 |
| Chromosome | 14 |
| Deletion Start | 31,168,489 |
| Deletion End | 31,139,128 |
| Deletion Size | 29,362 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 31,167,725 | 31,167,745 | CCCACCCAGTGTGCTGAGAAC |
| TUP | 31,167,699 | 31,167,723 | TGCTACAACCACTCACGAGGCCCTG |
| TUR | 31,167,666 | 31,167,685 | CCTAGGGACAGGCCCTCTTG |
| TDF | 31,139,782 | 31,139,801 | GGCTTTCAACGGCATCATTC |
| TDP | 31,139,733 | 31,139,757 | CCCATACTCACAGTCTGGGGAGGCA |
| TDR | 31,139,696 | 31,139,715 | TCATGCCTTTCCCCTTTGAG |
PCR Primers
| Primer | Sequence |
| SU | GTCGTCCATGTGGGGGTCAC |
| SD | ACTTCTCCCGGCGCCTCGTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 358 bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | 380 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
