10076   Dtx4     Status: ES cell colonies screened / QC no positives

GeneDtx4
Chromosome19
Deletion Start
Deletion End
Deletion Size
Genome Build37
MGI IDMGI:2672905
NCBI Gene ID207521
BAC ID407K22
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Sequence Downloads and Strategy Information

  1. 2,000 Bases Upstream (not yet available)
  2. 2,000 Bases Downstream (not yet available)
  3. Primer Sequences
  4. Cassette
  5. Design Diagram
  6. PCR Conditions
  7. Genotyping Strategy

Primer Genomic Coordinates on Chromosome 19

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF12,500,94612,500,964TCGTGGGTCCGCTGTGATC
TUP12,500,91312,500,938TGGCCAGCTTTGCTCACTGGTATCAG
TUR12,500,88612,500,906AGAAGCACCTGCCCTCAACAC
TDF12,469,70412,469,724CCCTTGTTGCCCTAGGTTCTG
TDP12,469,67812,469,702CGGTCCCAGGCCACTAGAAGCAGCT
TDR12,469,65312,469,673GGTTCCAATGGCAAAGATGAG

PCR Primers

PrimerSequence
SUCCGGGTGGCTTTGCAAGGCG
SDGCCAGGATATGGAATACCTC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR79 bp++-
TDFTDR72 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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