10082   Cd300lb     Status: ES cell colonies screened / QC one positive

GeneCd300lb
Chromosome11
Deletion Start
Deletion End
Deletion Size
Genome Build37
MGI IDMGI:2685099
NCBI Gene ID217304
BAC ID280C6
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Sequence Downloads and Strategy Information

  1. 2,000 Bases Upstream (not yet available)
  2. 2,000 Bases Downstream (not yet available)
  3. Primer Sequences
  4. Cassette
  5. Design Diagram
  6. PCR Conditions
  7. Genotyping Strategy

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUFGGGTATCCCGCTGAGATTTGG
TUPCTCCTTGATGAACAGCCAGCAGCAAA
TURGTCCTGCCTTCCCACTTTGC
TDFACTATTTAGAGGTGTGGCCTTGTTG
TDPAGCCCACACCCACAGTGACACACCT
TDRACTACCTCCATGAAGCTAGGAATAGG

PCR Primers

PrimerSequence
SUACACGAGCTGAGACTCTCAG
SDTGTTCCATCGATTCTCACCC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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