10086 Arhgef4 Status: ES cell colonies screened / QC no positives
| Gene | Arhgef4 |
| Chromosome | 1 |
| Deletion Start | 34,804,951 |
| Deletion End | 34,811,829 |
| Deletion Size | 6,879 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,805,492 | 34,805,513 | CTGCATCTGGTCACCAAAGAAC |
| TUP | 34,805,517 | 34,805,543 | TGATTCAGACTCCCAAAGGTCAGGCAG |
| TUR | 34,805,551 | 34,805,570 | ACCCGAGAACCAGGGAATTC |
| TDF | 34,811,706 | 34,811,725 | CCACAAGCATCCATCATTGC |
| TDP | 34,811,756 | 34,811,778 | CTTCCGCCTGGGCTCTGCTAGCA |
| TDR | 34,811,787 | 34,811,806 | TGCTGATGCTGTGCCAGAAG |
PCR Primers
| Primer | Sequence |
| SU | GGGGCCATGACAGTCAGTCC |
| SD | AGGTTTAGAGCTGTGGCGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 394 bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | 333 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
