10087 Dolk Status: Cancelled
| Gene | Dolk |
| Chromosome | 2 |
| Deletion Start | 30,286,028 |
| Deletion End | 30,284,427 |
| Deletion Size | 1,621 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,285,756 | 30,285,775 | TGCCTTTGCTTGGGCTAGTC |
| TUP | 30,285,732 | 30,285,754 | TGCAGTCTGGCAGCGCTCCTTCA |
| TUR | 30,285,706 | 30,285,724 | CCGAAGCGCTCGAAGTATG |
| TDF | 30,285,819 | 30,285,838 | GCAATGCCGTTTTCCAGTTC |
| TDP | 30,285,792 | 30,285,815 | ATGTCCGCGAACAGCGGCTTACTG |
| TDR | 30,285,761 | 30,285,779 | GCCCAAGCAAAGGCATGAC |
PCR Primers
| Primer | Sequence |
| SU | GAGGCGGTGGAGACTGTGAG |
| SD | AACCAGGAGAGCTTTGCTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 367 bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | 274 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
