10088 Fcrl1 Status: ES cell colonies screened / QC no positives
| Gene | Fcrl1 |
| Chromosome | 3 |
| Deletion Start | 87,376,477 |
| Deletion End | 87,391,291 |
| Deletion Size | 14,815 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 87,377,080 | 87,377,099 | GGCTCACCTGGCACCTTATC |
| TUP | 87,377,101 | 87,377,124 | CGATTCGGTTTCCACGTCACGTGA |
| TUR | 87,377,141 | 87,377,161 | CACGCATGAGTGGGAAAACAG |
| TDF | 87,390,366 | 87,390,386 | CCAGCCCCGTAGAGACAGAAC |
| TDP | 87,390,406 | 87,390,432 | CCAAAACGCCCTCATGCAGTGATTCTT |
| TDR | 87,390,435 | 87,390,454 | CGGGAAACGTACCCATAAGC |
PCR Primers
| Primer | Sequence |
| SU | TGAGTCATGCCATAGGAAGG |
| SD | ACCTTAGTTGCTTTCATCACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 362 bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | 375 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
