10089 Megf9 Status: ES cell colonies screened / QC no positives
Gene | Megf9 |
Chromosome | 4 |
Deletion Start | 70,488,323 |
Deletion End | 70,455,857 |
Deletion Size | 32,467 |
Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
Primer | Start | End | Sequence |
TUF | 70,488,297 | 70,488,319 | CTGTTCTGAGGTTGGAAGTTTGG |
TUP | 70,488,269 | 70,488,292 | AAAGCGCTGCAACCAGACCACAGG |
TUR | 70,488,239 | 70,488,261 | CCCTGATAACCCACATGACAATC |
TDF | 70,455,982 | 70,456,002 | AGTTCTGGGAATTGCCAATGC |
TDP | 70,455,949 | 70,455,973 | TGTCACCGGCTCCATGTGTGACAAA |
TDR | 70,455,923 | 70,455,945 | CCAAAGCCATAATGTCCATCTTG |
PCR Primers
Primer | Sequence |
SU | CACCCACTACATTGGCTTAG |
SD | AAGTGATTGTGGCTTGCTTG |
LacInR | TTGACTGTAGCGGCTGATGTTG |
LacInf | GGTAAACTGGCTCGGATTAGGG |
NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
NeoInF | TTCGGCTATGACTGGGCACAACAG |
LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
Forward Primer | Reverse Primer | Length | WT | HET | KO |
SU | LacZRev | 470 bp | - | + | + |
TUF | TUR | 81 bp | + | + | - |
TDF | TDR | 80 bp | + | + | - |
NeoFwd | SD | 381 bp | - | + | + |
LacInf | LacInR | 210 bp | - | + | + |
NeoInf | NeoInR | 282 bp | - | + | + |