10090 Tspan33 Status: ES cell colonies screened / QC no positives
| Gene | Tspan33 |
| Chromosome | 6 |
| Deletion Start | 29,694,427 |
| Deletion End | 29,717,638 |
| Deletion Size | 23,212 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 29,694,609 | 29,694,627 | CCTGAGGCTGGGAGGTTTG |
| TUP | 29,694,648 | 29,694,671 | CGAAGCACCACTCGACACGCTCCT |
| TUR | 29,694,679 | 29,694,698 | CCCAAGTGTCACCAGCTCAG |
| TDF | 29,716,886 | 29,716,906 | TGGGCAGAAAAGCTGTTATGG |
| TDP | 29,716,914 | 29,716,938 | CCTTCCCGAGCTTAACCAGGTGGCT |
| TDR | 29,716,944 | 29,716,965 | CGCAGTAGGGTGAAGACAAACC |
PCR Primers
| Primer | Sequence |
| SU | AGGAGAGGGATGGCTGGCTG |
| SD | TACAAACAGCATGAAATAAACCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 476 bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | 307 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
